Gynecologic Pathology
Mariem A Elfeky; Rema H Faraj Saad; Mohamed Ali Alabiad; Mohammed Alorini; Rehab Hemeda; Ramadan M Ali; Loay M Gertallah; Mohamed Negm; Ahmed Mahmoud Abdou; Ahmed Baker A Alshaikh; Ahmed Elmaasrawy
Abstract
Background: Cervical cancer spreads to the pelvic lymph nodes, leading to a high incidence of cancer recurrence and unfavorable survival rates. Therefore, there is an urgent need to detect new predictive biomarkers for the early assessment of pelvic lymph node status in patients with cervical cancer. ...
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Background: Cervical cancer spreads to the pelvic lymph nodes, leading to a high incidence of cancer recurrence and unfavorable survival rates. Therefore, there is an urgent need to detect new predictive biomarkers for the early assessment of pelvic lymph node status in patients with cervical cancer. Fatty acid binding protein 4 (FABP4) is an intracellular lipid chaperone that can carry fatty acids to many organelles. Subunit 2 of the GINS complex (GINS2), which belongs to the GINS complex family, encodes a protein that initiates DNA replication and controls the cell cycle and normal cell division. Chromobox homolog 7 (CBX7) was found to promote cancer occurrence and spread through the promotion of EMT.Objective: The current study aimed to assess the expression of FABP4, GINS2, and CBX7 in cancer cervix tissue to detect their prognostic and predictive roles in the development of lymph node metastases in cancer cervix patientsMethods: we collected tissues from patients with cancer cervix and evaluated the expression of FABP4, GINS2, and CBX7 using immunohistochemistry. We evaluated the association between their expression and clinicopathological and prognostic parameters.Results: high expression of FABP4 and GINS2, in addition to low expression of CBX7, was positively associated with the old age group, large tumor size, high grade and lymphovascular involvement, para-uterine organ infiltration, advanced FIGO stage, chemotherapeutic resistance, and tumor recurrence.Conclusion: We demonstrated the oncogenic roles of FABP4 and GISN2 in addition to the on-co-suppressive roles of CBX7 in cervical cancer and their association with poor clinicopathological criteria and poor survival. Our results indicated that FABP4, GISN2, and CBX7 could be considered predictive biomarkers of the occurrence of lymph node metastases in the cancer cervix preoperatively, which could be beneficial in the accurate preoperative design therapy.
Uropathology
Walid S. H. Elsayed; Ola Harb; Mohamed A Alabiad; Rema H. Faraj Saad; Amal Anbaig; Mohamed Alorinin; Rehab Hemeda; Mohamed Negm; Loay M Gertallah; Walled A Abdelhady; Ramadan M Ali
Abstract
Background & Objective: Cells of renal cell carcinoma (RCC) are resistant to the most currently used chemotherapeutic agents and targeted therapies; hence, we evaluated the expression of NEK2, JMJD4, and REST in tissues of clear cell renal cell carcinoma (ccRCC) and benign nearby tissues of kidney ...
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Background & Objective: Cells of renal cell carcinoma (RCC) are resistant to the most currently used chemotherapeutic agents and targeted therapies; hence, we evaluated the expression of NEK2, JMJD4, and REST in tissues of clear cell renal cell carcinoma (ccRCC) and benign nearby tissues of kidney with the aim of detecting associations between their expression and clinicopathological features, prognostic data, tumor recurrence, and survival rates.Methods: We collected 200 samples from tumor and adjacent non-neoplastic tissues of 100 ccRCC patients. All samples were evaluated for the expression of NEK2, JMJD4, and REST, and the patients were followed up for about 5 years. Tumor recurrence and survival data were collected and analyzed.Results: NEK2 and JMJD4 expression was increased in ccRCC tissues (P=0.002 and 0.006), while REST was downregulated (P<0.001). The elevated expression of NEK2 was positively related with big tumor size (P=0.015), higher grades (P=0.002), higher stages (P=0.013), distant spread (P=0.004), tumor recurrence, shorter progression-free survival (PFS) rate, and overall survival (OS) rate (P<0.001). Likewise, the high expression of JMJD4 was positively related with big tumor size (P=0.047), higher grades (P=0.003), higher stages (P=0.043), distant spread (P=0.001), tumor recurrence, shorter PFS rate, and OS rate (P<0.001). Conversely, Low expression of REST was positively related to big tumor size, higher grades, higher stages, distant spread, tumor recurrence, and shorter PFS and OS rates (P<0.001).Conclusion: We demonstrated that overexpression of NEK2 and JMJD4 and downregulation of REST were found in malignant than benign renal tissues and were related to unfavorable pathological findings, poor clinical parameters, and poor patient outcomes.
